Journal: Journal of functional foods

Article Title: Efficacy comparison of lyophilised black raspberries and combination of celecoxib and PBIT in prevention of carcinogen-induced oesophageal cancer in rats

doi: 10.1016/j.jff.2016.08.044

Figure Lengend Snippet: In-vitro mechanistic study of black raspberry anthocyanins, anthocyanin metabolites, celecoxib and S,S′-1,4-phenylene-bis(1,2-ethanediyl)bis-isothiourea (PBIT) in human esophageal squamous cell carcinoma KYSE-270 cells. A to D, WST-1 analysis of the growth of KYSE-270 cells that were treated with cyaniding-3-rutinosides (C3R), cyanidin-3-glucoside (C3G), cyaniding-3-sambubioside (C3S), pelargonidin-3-rutinoside (P3R), protocatechuic acid (PCA), 4-hydroxybenzoic acid (4HBA), celecoxib, PBIT or celecoxib + PBIT; E, Western blot analysis of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and phosphorylation of extracellular signal-regulated kinases (ERK) and AKT modulated by C3R, celecoxib, PBIT or celecoxib + PBIT; and F, Western blot analysis of COX-2, iNOS, and phosphorylation of ERK and AKT modulated by C3R, celecoxib, PBIT or PCA at dose of IC50 value. Samples were probed with antibodies against COX-2, iNOS, p-ERK or p-AKT. The Western blot membranes were stripped and reprobed for GADPH as an internal control to confirm equal loading. Representative blots were from one of three separate experiments. The values are expressed as mean; bars, ± SE; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The anthocyanin cyanidin-3-glucoside (C3G), cyanidin-3-rutinoside (C3R), cyaniding-3-sambubioside (C3S), pelargonidin-3-rutinoside (P3R), anthocyanin metabolite protocatechuic acid (PCA) and 4-hydroxybenzoic acid (4HBA) were purchased from Indofine Chemical Company (Hillsborough, NJ).

Techniques: In Vitro, Western Blot, Phospho-proteomics, Control

Journal: Cell

Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

doi: 10.1016/j.cell.2019.10.035

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner TM (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, Purification, Labeling, Plasmid Preparation, Software